PerfeCTa qPCR SuperMix
Features & Benefits
Product
Kit Size
Part Number
Price
Quantity
PerfeCTa qPCR SuperMix
Kit Size:
500 x 50 μL rxns (10 x 1.25 mL)
Part Number:
95050-500
1066.00
Kit Size:
2000 x 50 μL rxns (1 x 50 mL)
Part Number:
95050-02K
2691.00
PerfeCTa qPCR SuperMix ROX
Kit Size:
500 x 50 μL rxns (10 x 1.25 mL)
Part Number:
95051-500
1066.00
Kit Size:
2000 x 50 μL rxns (1 x 50 mL)
Part Number:
95051-02K
2691.00
PerfeCTa qPCR SuperMix Low-ROX
Kit Size:
500 x 50 μL rxns (10 x 1.25 mL)
Part Number:
95052-500
1066.00
Kit Size:
2000 x 50 μL rxns (1 x 50 mL)
Part Number:
95052-02K
2691.00
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Description
PerfeCTa qPCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers, probe(s), and template for real-time quantitative PCR systems. The proprietary buffer and stabilizers have been specifically optimized to deliver maximum PCR efficiency, sensitivity, and robust fluorescent signal with TaqMan® or TaqMan MGB probe chemistry. The enhanced specificity of this supermix suppresses cross-reactivity between homologous sequences, improving detection and discrimination in SNP applications. A key component of this supermix is AccuStart Taq DNA polymerase, which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (2 minutes at 95ºC), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.
Details
Contents
Storage & Handling
PerfeCTa qPCR SuperMix (2X): 2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), AccuStart Taq DNA Polymerase, and stabilizers.
Instrument Capability
ROX
- Applied Biosystems 5700
- Applied Biosystems 7000
- Applied Biosystems 7300
- Applied Biosystems 7700
- Applied Biosystems 7900
- Applied Biosystems 7900HT
- Applied Biosystems 7900 HT Fast
- Applied Biosystems StepOne™
- Applied Biosystems StepOnePlus™
Low ROX
- Applied Biosystems 7500
- Applied Biosystems 7500 Fast
- Stratagene Mx3000P®
- Stratagene Mx3005P™
- Stratagene Mx4000™
- Applied Biosystems ViiA 7
- Applied Biosystems QuantStudio™
- Agilent AriaMx
- Douglas Scientific IntelliQube®
No ROX
- Quantabio Q
- BioRad CFX
- Roche LightCycler 480
- QIAGEN Rotor-Gene Q
- Other
Bio-Rad iCycler iQ systems
- BioRad iCycler iQ™
- BioRad MyiQ™
- BioRad iQ™5
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