PerfeCTa SYBR Green SuperMix
Sensitive and precise DNA amplification with DNA-intercalating dye based detection chemistry
Features & Benefits
- 2x concentrated reagents minimize pipetting steps, simplify reaction assembly and improve accuracy
- Superior assay sensitivity and specificity with ultrapure AccuStart enzyme technology – maximum-yielding Taq DNA polymerase mutant controlled by stringent, multi-epitope antibody hot start
- Supports efficient vortex mixing with proprietary anti-foaming formulation
- SuperMix version provides maximum dye concentration for robust optical signal with small amplicons (i.e. microRNAtemplated cDNA)
PerfeCTa SYBR Green SuperMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
Product
Kit Size
Order Info
PerfeCTa SYBR Green SuperMix for iQ
500 x 50 μL rxns (10 x 1.25 mL)
2000 x 50 μL rxns (1 x 50 mL)
PerfeCTa SYBR Green SuperMix
500 x 50 μL rxns (10 x 1.25 mL)
2000 x 50 μL rxns (1 x 50 mL)
PerfeCTa SYBR Green SuperMix ROX
500 x 50 μL rxns (10 x 1.25 mL)
2000 x 50 μL rxns (1 x 50 mL)
PerfeCTa SYBR Green SuperMix Low ROX
500 x 50 μL rxns (10 x 1.25 mL)
2000 x 50 μL rxns (1 x 50 mL)
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Description
PerfeCTa SYBR Green SuperMix is a user-friendly, 2X concentrated reaction mix that simplifies setup and is pre-blended with reference dye for optimized product performance. This proprietary buffer technology stabilizes a high concentration of SYBR Green I dye to ensure maximum optical signal with low abundance or small targets (such as microRNA). Successful detection with a non-specific, dsDNA intercalating dye requires precise target amplification as off-target primer elongation will contribute to overall fluorescent signal and lead to over-reported relative abundance values. This reagent is powered by a highly-processive, ultra-pure Taq DNA polymerase mutant with stringent, ultra-pure AccuStartII antibody hot start technology that allows ambient room-temperature setup and maximal enzyme kinetics after rapid, irreversible denaturation at 95°C.
Details
Contents
Storage & Handling
Single-tube, 2X concentrated reagent containing:
- Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, and dTTP.
- AccuStart II Taq DNA Polymerase
- SYBR Green I dye
- Proprietary enzyme stabilizers and performance-enhancing additives.
- Titrated reference dye (if applicable).
Instrument Capability
ROX
- Applied Biosystems 5700
- Applied Biosystems 7000
- Applied Biosystems 7300
- Applied Biosystems 7700
- Applied Biosystems 7900
- Applied Biosystems 7900HT
- Applied Biosystems 7900 HT Fast
- Applied Biosystems StepOne™
- Applied Biosystems StepOnePlus™
Low ROX
- Applied Biosystems 7500
- Applied Biosystems 7500 Fast
- Stratagene Mx3000P®
- Stratagene Mx3005P™
- Stratagene Mx4000™
- Applied Biosystems ViiA 7
- Applied Biosystems QuantStudio™
- Agilent AriaMx
- Douglas Scientific IntelliQube®
No ROX
- Quantabio Q
- BioRad CFX
- Roche LightCycler 480
- QIAGEN Rotor-Gene Q
- Other
Bio-Rad iCycler iQ systems
- BioRad iCycler iQ™
- BioRad MyiQ™
- BioRad iQ™5
Performance Data
PerfeCTa SYBR Green SuperMix Comparison to QuantiTect SYBR Green PCR Kit (Qiagen), GAPD
Log-fold serial dilutions of qScript™ cDNA from HeLa cell total RNA (100 ng to 0.1 pg) were analyzed by SYBR Green qPCR following each manufacturers protocol. Averaged plots of triplicate qPCR reactions are shown.
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