First-Strand cDNA Synthesis
Quantabio reverse transcription reagents leverage proprietary, performance-engineered qScript® reverse transcriptase in a variety of stabilized, user-friendly reagent formulations that maximize cDNA yield and provide linear cDNA synthesis across a broad dynamic range of input RNA. qScript cDNA synthesis kits redefine what is possible in speed, convenience, reproducibility, specificity and limit of detection (LOD) sensitivity in qPCR and RT-PCR applications.
First-Strand cDNA Synthesis comparison chart
qScript cDNA SuperMix | qScript XLT cDNA SuperMix | qScript cDNA Synthesis Kit | qScript Flex cDNA Synthesis Kit | qScript Ultra Flex Kit | |
---|---|---|---|---|---|
Features | |||||
Kit Format | One Tube | One Tube | Two Tubes | Six Tubes (water included) | Five Tubes (water included) |
RT Enzyme | MMLV, RNase H+ | MMLV, reduced RNase activity | MMLV, RNase H+ | MMLV, RNase H+ | qScript Ultra RT |
Concentration | 5x | 5x | 5x | 5x | 5x |
Yield | +++ | ++++ | ++ | ++ | **** |
Total RNA Input Range | 10pg – 1ug | 1pg – 1ug | 10pg – 1ug | 10pg – 4ug | 1pg – 2.5ug |
Amplicon Length | 1 kb or less | 1 kb or less | 1 kb or less | 12 kb or less | 20 kb or less |
Optimal Reaction Time | 40 min | 30 - 70 min | 40 min | 60 - 90 min | 10 min |
Priming Method | |||||
Oligo(dt) | x | x | x | x | x |
Random | x | x | x | x | x |
Gene Specific | x | x | |||
Applications | |||||
High Yield | x | x | x | ||
Available Formats | |||||
Master Mix | x | x | |||
Packaging (Rxns/Units) | 25 Rxns 100 Rxns 500 Rxns | 25 Rxns 100 Rxns 500 Rxns | 25 Rxns 100 Rxns 500 Rxns | 25 Rxns 100 Rxns | 25 Rxns 100 Rxns |
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Reverse Transcription Products
Showing all 5 results
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qScript cDNA SuperMix
qScript cDNA SuperMix is a sensitive and easy-to-use 1-tube reagent for first-strand cDNA synthesis that combines a highly-modified RNAse H+ mutant of M-MLV together with ribonuclease inhibitor protein (RIP) in a rigorously optimized formulation for real-time qPCR applications. The stabilized…Debugging in WordPress for more information. (This message was added in version 3.0.) in /var/www/vhosts/quantabio-wp.perrill.dev/httpdocs/wp-includes/functions.php on line 5865
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qScript cDNA Synthesis Kit
The qScript cDNA Synthesis Kit is a sensitive and easy-to-use solution for RNA quantification using two step RT-qPCR. The novel qScript Reaction Mix provides all the necessary components for cDNA synthesis except enzyme and RNA template. qScript reverse transcriptase is…Debugging in WordPress for more information. (This message was added in version 3.0.) in /var/www/vhosts/quantabio-wp.perrill.dev/httpdocs/wp-includes/functions.php on line 5865
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qScript Flex cDNA Kit
The qScript Flex cDNA Synthesis Kit is configured in separate components for supporting multiple RT priming strategies. The kit provides optimized, flexible components for priming with oligo-dT(20), random hexamers, gene-specific primer (GSP), or any combination thereof. This unique 5X master…Debugging in WordPress for more information. (This message was added in version 3.0.) in /var/www/vhosts/quantabio-wp.perrill.dev/httpdocs/wp-includes/functions.php on line 5865
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qScript Ultra Flex Kit
qScript Ultra Flex Kit is easy-to-use and highly efficient for the synthesis of first-strand cDNA with flexible priming methods including oligo-dT, random hexamer and gene specific priming to reverse transcribe RNA to cDNA.Debugging in WordPress for more information. (This message was added in version 3.0.) in /var/www/vhosts/quantabio-wp.perrill.dev/httpdocs/wp-includes/functions.php on line 5865
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qScript XLT cDNA SuperMix
qScript XLT cDNA SuperMix is a next-generation tool for first-strand cDNA synthesis, providing a highly sensitive and easy-to-use solution for two-step RT-PCR and RT-qPCR. qScript XLT is an engineered M-MLV reverse transcriptase mutant with reduced RNase H activity and improved…Debugging in WordPress for more information. (This message was added in version 3.0.) in /var/www/vhosts/quantabio-wp.perrill.dev/httpdocs/wp-includes/functions.php on line 5865
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