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PCR & qPCR - Quantabio
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PCR & qPCR
PCR / qPCR

Specific & Efficient Primer Extension

Non-specific extension of primers at low temperature is a common cause of PCR artifacts and poor assay specificity and sensitivity. That’s why Quantabio PCR reagents are formulated with ultrapure AccuStart™ DNA polymerase, which contains a stringent antibody hot start to ensure specific and efficient primer extension only after activation at 94°C.

Our enzymes are rigorously purified to remove host E. coli genomic DNA and are ideally suited for applied testing applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy targets.

Quantabio manufactured reagents:

  • Maximize assay performance
  • Minimize errors
  • Deliver reliable, reproducible results
Workflow Solutions

Cells and tissues

Cells and tissues

DNA Extraction

DNA Extraction

Amplification

Amplification
Detection
Detection
Customer Testimonials
repliQa HiFi ToughMix

"Worked great amplifying two gene target from plasmid DNA. It was the first time I have performed a two step reaction and it worked great. It was awesome running everything in 23 minutes too."

Dr. Brandon L. | University of Nebraska
repliQa HiFi ToughMix

"Worked great amplifying two gene target from plasmid DNA. It was the first time I have performed a two step reaction and it worked great. It was awesome running everything in 23 minutes too."

Dr. Brandon L. | University of Nebraska
repliQa HiFi ToughMix

"I used NEB Q5 for the same plasmid for PCR, same annealing temperature. Only the repliQa HiFi toughMix gave me the correct size products. It is very fast and works much better than NEB Q5 for difficult plasmids in PCR."

Dr. Kara C. | UT Southwestern Medical Center
AccuStart Long Range SuperMix

"The AccuStart Long Range SuperMix produced a clean long-range product (10 kbp) on our first try! We are excited to have a reliable and easy-to-use polymerase to produce DNA for single-molecule studies."

Danielle M. | Professor, Stanford University
PerfeCTA SYBR Green FastMix

"With the PerfeCTa SYBR Green FastMix we had less variability in our triplicates and more reproducible results between repeat experiments."

With the PerfeCTa SYBR Green FastMix we had less variability in our triplicates and more reproducible results between repeat experiments. | Professor, UCSF
PerfeCTA SYBR Green SuperMix

"PerfeCTa SYBR Green SuperMix has been my first and successful experience that boosted my confidence for qPCR. I am really satisfied with its performance."

Teaching Assistant | Ohio University
repliQa HiFi ToughMix

"This can amplify the tough template for SURE. I was struggled with the tough template that showed poor quality results with other many PCR enzymes. This repliQa saved my life and eventually gave me the clear and strong band by gel."

Lili C. | Post Doc, USC
repliQa HiFi ToughMix

"The kit have a great power of amplification and extreme fast speed, I just need one hour to get result which I usually spent three hours before."

Simon S. | Professor, Tennessee State University

PCR Comparisons

AccuStart II PCR SuperMixAccuStart II GelTrack PCR SuperMixsparQ HiFi PCR Master MixAccuStart II PCR ToughMixrepliQa HiFi ToughMixAccuStart Long Range SuperMix
Features

Concentration

2x

2x

2x

2x

2x

4x

Amplicon Size

Up to 4 kb

Up to 4 kb

Up to 4 kb

Up to 4 kb

Up to 24 kb

Up to 24 kb

Extension Time*

60 sec/kb

60 sec/kb

30 sec/kb

60 sec/kb

1-10 sec/kb

30-60 sec/kb

Multiplex PCR

Up to 6 Targets

Fidelity vs. Taq

1x

1x

80x Taq

1x

90x Taq

10-12x Taq

dU Tolerant

No

No

No

No

Yes

Yes

5′ – 3′ exo

x

x

N/A

x

N/A

N/A

3′ – 5′ exo

N/A

N/A

x

N/A

x

x

Resulting Ends

3′ dA overhang

3′ dA overhang

Blunt

3′ dA overhang

Blunt

Blunt/T overhangs

Applications

Routine PCR

x

x

x

x

x

High Fidelity

x

**

x

Crude PCR

x

**

Fast PCR

**

Long PCR

x

**

High Yield

x

x

**

x

NGS Library Amplification

**

x

Available Formats

Hot Start

x

x

x

x

x

x

Master Mix

x

x

x

x

x

x

Loading Dye Available

x

x

Packaging (Rxns/Units)

100 Rxns
500 Rxns
4000 Rxns

100 Rxns
500 Rxns
4000 Rxns

50 Rxns
250 Rxns

100 Rxns
800 Rxns
4000 Rxns

25 Rxns
100 Rxns
500 Rxns

25 Rxns
100 Rxns

qPCR Comparisons

PerfeCTa SYBR Green SuperMixPerfeCTA SYBR Green FastMixAccuStart Genotyping ToughMixPerfeCTa qPCR FastMix UNGPerfeCTa FastMix IIPerfeCTa qPCR ToughMixPerfeCTa qPCR ToughMix, UNGPerfeCTa Multiplex qPCR ToughMixPerfeCTa Multiplex qPCR SuperMix
Features

Concentration

2x

2x

2x

2x

2x

2x

2x

5x

2x

Sample Type

gDNA, cDNA

gDNA, cDNA

gDNA, cDNA

gDNA, cDNA

gDNA, cDNA

gDNA, cDNA

gDNA, cDNA

gDNA, cDNA

gDNA, cDNA

Fast Cycling

No

Yes

Yes

Yes

Yes

Yes

Yes

Yes

Yes

Multiplex Compatibility

N/A

N/A

Up to 2 targets

Up to 2 targets

Up to 2 targets

Up to 2 targets

Up to 2 targets

Up to 5 targets

Up to 4 targets

Carryover Contamination Control

N/A

N/A

N/A

Heat-labile UNG & blend of dTTP/dUTP

N/A

N/A

Heat-labile UNG & blend of dTTP/dUTP

N/A

N/A

Applications

Gene Expression

x

x

x

x

x

x

x

x

microRNA

x

x

ChIP

x

x

SNP Genotyping

x

Inhibitor Tolerant

x

**

x

x

High Yield

x

x

x

**

Available Formats

Master Mix

x

x

x

x

x

x

x

x

x

Packaging (Rxns/units)

250 Rxns
1250 Rxns
2000 Rxns

250 Rxns
1250 Rxns
5000 Rxns

250 Rxns
1250 Rxns
5000 Rxns

250 Rxns
1250 Rxns
5000 Rxns

250 Rxns
1250 Rxns
5000 Rxns

250 Rxns
1250 Rxns
5000 Rxns

250 Rxns
1250 Rxns
5000 Rxns

250 Rxns
1250 Rxns
5000 Rxns

50 Rxns
200 Rxns
1000 Rxns

We’re Here to Help

Do you have questions about our products or their applications? Our experts are standing by for support.

Contact Us

Product Finder

Select Your Assay

Starting Template

Assay Format

Detection Chemistry

Multiplexing (more than 3 targets)

Is gene-specific priming (GSP) required?

What current Reverse Transcriptase or cDNA kit are you using?

Select the group which contains your real-time PCR cycler

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™
  • Agilent AriaMx
  • Douglas Scientific IntelliQube®
  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™
  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Other
  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5

Choose your application from the categories below

Products

I give Quantabio or an authorized Quantabio distributor permission to contact me for product updates and news.
* Required information

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