"The Perfecta Multiplex qPCR ToughMix greatly simplified my qPCR assay by removing DNA isolation steps and complexity of passive reference dyes. I will definitely be using this product in the future."

"This eliminates a costly and time consuming step to remove inhibitors manually, so it has saved us money on reagents and time for our small lab."

"Higher sensitivity, positives at a lower Cq. Great results with plant DNA with high inhibition level."

"Compared to other 1-step qRT-PCR mixes we have used, UltraPlex 1-Step ToughMix (4X) Low-ROX, 500R performs with the same sensitivity when testing using SARS-CoV-2-spiked saliva samples, but is easier to use and at a significantly lower price per reaction. The mix requires little to no time to thaw, decreasing the time required when preparing samples. The mix is also conveniently aliquoted into multiple tubes, reducing the chance for contamination as opposed to a single, high volume aliquot. Overall, these factors make this a better overall product as compared to competing companies (e.g., Thermo Fisher Scientific or Zymo) and we will continue to use this moving forward."

"1-Step Virus ToughMix performed BETTER than other 3 mixes and amplified very well the S target from SARS-CoV-2 in a highly multiplex environment."

"It is an easy kit that contains all the reagents needed for the master mix in one tube. The only components I added were my primers, probe and nuclease free water. The protocol is short and user friendly."