qScript XLT 1-Step RT-qPCR ToughMix
Tough-tested 1-step real time PCR
Features & Benefits
- Tough Tested – Overcomes common inhibitors including polysaccharides, heme/hemoglobin, humic acid, melanin
- Flexible – Use fast or standard qPCR cycling conditions
- Broad dynamic range- Reliable data from your precious samples every time
- Multiplexing enabled – Supports highly sensitive detection for up to four targets
qScript XLT 1-Step RT-qPCR ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
Product
Kit Size
Part Number
Price
Quantity
qScript XLT 1-Step RT-qPCR ToughMix ROX
Kit Size:
100 x 20 μL rxns (1 x 1 mL)
Part Number:
95133-100
189.00
Kit Size:
500 x 20 μL rxns (5 x 1 mL)
Part Number:
95133-500
652.00
Kit Size:
2000 x 20 μL rxns (1 x 20 mL)
Part Number:
95133-02K
2488.00
qScript XLT 1-Step RT-qPCR ToughMix Low-ROX
Kit Size:
100 x 20 μL rxns (1 x 1 mL)
Part Number:
95134-100
189.00
Kit Size:
500 x 20 μL rxns (5 x 1 mL)
Part Number:
95134-500
652.00
Kit Size:
2000 x 20 μL rxns (1 x 20 mL)
Part Number:
95134-02K
2488.00
qScript XLT 1-Step RT-qPCR ToughMix
Kit Size:
100 x 20 μL rxns (1 x 1 mL)
Part Number:
95132-100
188.00
Kit Size:
500 x 20 μL rxns (5 x 1 mL)
Part Number:
95132-500
652.00
Kit Size:
2000 x 20 μL rxns (1 x 20 mL)
Part Number:
95132-02K
2488.00
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Description
qScript XLT One-Step RT-qPCR ToughMix is a ready-to-use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan® 5'-hydrolysis probes. First-strand cDNA synthesis and subsequent PCR amplification are carried out seamlessly in the same reaction mixture with optimized 1-step thermal cycling parameters.
This kit is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets as well as high throughput gene-expression studies. The system has been optimized to deliver maximum RT-PCR efficiency, sensitivity, and specificity in minimal reaction volumes and accelerated thermal cycling rates. The only necessary user-supplied materials for RT-qPCR is RNA sample and probe assay. It is compatible with all types of molecular probe assays including dual-labeling strategies.
Elevating cDNA synthesis reaction temperature to 50-55°C during one-step RT-qPCR improves primer annealing and disruption of RNA secondary structure that can interfere with cDNA synthesis.
Details
Contents
Storage & Handling
2X concentrated One-Step Master Mix containing:
- Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, dTTP
- qScript XLT reverse transcriptase
- RNase inhibitor protein
- AccuStart II Taq DNA Polymerase
- Inert AccuVue™ plate loading dye
- Proprietary enzyme stabilizers and performance-enhancing additives
- Titrated reference dye (if applicable)
Instrument Capability
ROX
- Applied Biosystems 5700
- Applied Biosystems 7000
- Applied Biosystems 7300
- Applied Biosystems 7700
- Applied Biosystems 7900
- Applied Biosystems 7900HT
- Applied Biosystems 7900 HT Fast
- Applied Biosystems StepOne™
- Applied Biosystems StepOnePlus™
Low ROX
- Applied Biosystems 7500
- Applied Biosystems 7500 Fast
- Stratagene Mx3000P®
- Stratagene Mx3005P™
- Stratagene Mx4000™
- Applied Biosystems ViiA 7
- Applied Biosystems QuantStudio™
- Agilent AriaMx
- Douglas Scientific IntelliQube®
No ROX
- Quantabio Q
- BioRad CFX
- Roche LightCycler 480
- QIAGEN Rotor-Gene Q
- Other
Bio-Rad iCycler iQ systems
- BioRad iCycler iQ™
- BioRad MyiQ™
- BioRad iQ™5