US

UltraPlex 1-Step ToughMix

Tough-tested, multiplex, 1-step real time PCR
Features & Benefits
  • 4X concentrated master mix allows greater flexibility with dilute samples – as little as 1 pg of total RNA
  • Multiplexing capable with up to 5 targets
  • Tough-tested for applications where PCR inhibition from template impurities may be a concern, such as: crude extracts, clinical specimens, or environmental samples.
  • Temperature stabilized master mix enables convenient reaction setup at ambient room temperature

 

UltraPlex 1-Step ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

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Part Number
Price
Quantity
 
Product
Kit Size
Order Info
Price
Quantity

UltraPlex 1-Step ToughMix

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Part Number:
Price:
Quantity:
Kit Size:  100 x 20 µL rxns (1 x 500 µL)
Part Number:  95166-100
208.00
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Kit Size:  500 x 20 µL rxns (5 x 500 µL)
Part Number:  95166-500
908.00
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Kit Size:  1000 x 20 µL rxns (1 x 5 mL)
Part Number:  95166-01K
1721.00
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UltraPlex 1-Step ToughMix ROX

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Kit Size:
Part Number:
Price:
Quantity:
Kit Size:  100 x 20 µL rxns (1 x 500 µL)
Part Number:  95167-100
208.00
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UltraPlex 1-Step ToughMix Low ROX

Request Sample
Kit Size:
Part Number:
Price:
Quantity:
Kit Size:  500 x 20 µL rxns (5 x 500 µL)
Part Number:  95168-500
908.00
Add to Cart
Kit Size:  100 x 20 µL rxns (1 x 500 µL)
Part Number:  95168-100
208.00
Add to Cart
Kit Size:  1000 x 20 µL rxns (1 x 5 mL)
Part Number:  95168-01K
1721.00
Add to Cart
To place your order through VWR, click here

Description

UltraPlex 1-Step ToughMix is a ready-to-use, 4X concentrated master mix 1-step reverse transcription and real-time quantitative PCR (RT-qPCR) of RNA templates using probe-based detection methods. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between steps. Optimized to deliver highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or highly multiplexed RNA detection assays, this reagent chemistry is optimized to deliver maximum assay sensitivity, precision and reproducibility with miniaturized reaction volumes and either conventional or accelerated thermal cycling protocols. UltraPlex 1-Step ToughMix contains all required components for RT-qPCR except RNA template and probe and is compatible with all dual-labeled probe chemistries.

Webinar

SalivaDirectTM: A simplified and flexible platform to enhance SARS-CoV-2 testing capacity

Dr. Anne Wyllie
 Epidemiologist, Yale School of Public Health

 

 

 

What Customers Say

Compared to other 1-step qRT-PCR mixes we have used, UltraPlex 1-Step ToughMix (4X) Low-ROX, 500R performs with the same sensitivity when testing using SARS-CoV-2-spiked saliva samples, but is easier to use and at a significantly lower price per reaction. The mix requires little to no time to thaw, decreasing the time required when preparing samples. The mix is also conveniently aliquoted into multiple tubes, reducing the chance for contamination as opposed to a single, high volume aliquot. Overall, these factors make this a better overall product as compared to competing companies (e.g., Thermo Fisher Scientific or Zymo) and we will continue to use this moving forward.

Professor of Infectious Diseases UF Eminent Scholar DIRECTOR, CDC Southeastern Regional Center of Excellence for Vector Borne Diseases
Rhoel R. D., PhD, MPH
Details

Details

Contents

UltraPlex 1-Step ToughMix (Low ROX/ ROX) (4X), contains:

  • 4X reaction buffer containing optimized titrations of qPCR-grade dATP, dCTP, dGTP, dTTP, and magnesium.
  • Ultra-pure recombinant proteins: qScript XLT reverse transcriptase, RNase inhibitor protein, and AccuStart II hot-start Taq DNA polymerase.
  • Proprietary stabilizers additives and optimized ROX passive reference dye (if applicable).
Instrument Capability

Instrument Capability

ROX

  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™

Low ROX

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™
  • Agilent AriaMx
  • Douglas Scientific IntelliQube®

No ROX

  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Other

Bio-Rad iCycler iQ systems

  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5
Resource Panel
Customer Testimonials

Customer Testimonials

UltraPlex 1-Step ToughMix

"In side-by-side comparison, QuantaBio UltraPlex 1-Step ToughMix out performed ABI TaqPath, ABI TaqMan FastVirus, and ABI AgPath-ID One-Step RT-PCR kits."

Sladana B. | Plant Disease Diagnostician
UltraPlex 1-Step ToughMix

"Compared to other 1-step qRT-PCR mixes we have used, UltraPlex 1-Step ToughMix (4X) Low-ROX, 500R performs with the same sensitivity when testing using SARS-CoV-2-spiked saliva samples, but is easier to use and at a significantly lower price per reaction. The mix requires little to no time to thaw, decreasing the time required when preparing samples. The mix is also conveniently aliquoted into multiple tubes, reducing the chance for contamination as opposed to a single, high volume aliquot. Overall, these factors make this a better overall product as compared to competing companies (e.g., Thermo Fisher Scientific or Zymo) and we will continue to use this moving forward."

Rhoel R. D., PhD, MPH Professor of Infectious Diseases UF Eminent Scholar Director | CDC Southeastern Regional Center of Excellence for Vector Borne Diseases

Details

Contents

UltraPlex 1-Step ToughMix (Low ROX/ ROX) (4X), contains:

  • 4X reaction buffer containing optimized titrations of qPCR-grade dATP, dCTP, dGTP, dTTP, and magnesium.
  • Ultra-pure recombinant proteins: qScript XLT reverse transcriptase, RNase inhibitor protein, and AccuStart II hot-start Taq DNA polymerase.
  • Proprietary stabilizers additives and optimized ROX passive reference dye (if applicable).

Instrument Capability

ROX

  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™

Low ROX

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™
  • Agilent AriaMx
  • Douglas Scientific IntelliQube®

No ROX

  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Other

Bio-Rad iCycler iQ systems

  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5

Resources

Flyers
Flyer UltraPlex One-Step ToughMix

Product Manuals
Manual UltraPlex 1-Step ToughMix Low ROX (4X)
Manual Ultraplex 1-Step ToughMix, ROX
Manual Ultraplex 1-Step ToughMix

Application Notes
Application Note High Capacity COVID-19 testing

CofA (PSFs)
PSF-95166-01K-LOT66165319.pdf
PSF-95168-500-LOT66168576.pdf
PSF-95166-500-LOT66168674.pdf
PSF-95166-100-LOT2366158195.pdf
PSF-95166-100-LOT66168670.pdf
PSF-95166-100-LOT66158195.pdf
PSF-95166-500-LOT66157160.pdf
PSF-95168-100-LOT66157825.pdf
PSF-95168-500-LOT66157129.pdf
PSF-95166-100-LOT66155755.pdf
PSF-95166-500-LOT66155754.pdf
PSF-95167-100-LOT66155757.pdf
PSF-95168-100-LOT66155955.pdf
PSF-95168-500-LOT66155760.pdf
PSF-95166-100-LOT66154306.pdf
PSF-95168-500-LOT66154460.pdf
PSF-95166-500-LOT66153258.pdf
PSF-95168-100-LOT66153260.pdf
PSF-95166-01K-LOT66152573.pdf
PSF-95166-01K-LOT66148967.pdf
PSF-95166-500-LOT2366148043.pdf
PSF-95166-500-LOT66148043.pdf
PSF-95166-01K-LOT66147370.pdf
PSF-95168-100-LOT66141489.pdf
PSF-95168-500-LOT66141490.pdf
PSF-95166-100-LOT66142496.pdf
PSF-95166-500-Lot23028737.pdf
PSF-95166-100-LOT66140414.pdf
PSF-95166-01K-LOT66139974.pdf
PSF-95168-500-LOT66139972.pdf
PSF-95166-100-Lot030094.pdf
PSF-95168-100-Lot030046.pdf
PSF-95166-500-Lot030294.pdf
PSF-95167-500-Lot030312.pdf
PSF-95167-100-Lot030000.pdf
PSF-95167-500-Lot030001.pdf
PSF-95166-01K-Lot029948.pdf
PSF-95167-100-Lot029689.pdf
PSF-95168-100-Lot029530.pdf
PSF-95168-500-Lot029531.pdf
PSF-95166-500-Lot028737.pdf
PSF-95168-500-Lot028877.pdf
PSF-95166-500-Lot028185.pdf
PSF-95168-500-Lot027906.pdf
PSF-95166-100-Lot027588.pdf
PSF-95166-500-Lot027589.pdf
PSF-95168-100-Lot027045.pdf
PSF-95168-100-Lot026975.pdf
PSF-95166-100-Lot026369.pdf
PSF-95166-500-Lot026370.pdf
PSF-95168-500-Lot025801.pdf
PSF-95167-100-Lot025466.pdf
PSF-95166-100-Lot025390.pdf
PSF-95166-500-Lot025391.pdf
PSF-95168-500-Lot025386.pdf
PSF-95168-500-Lot024522.pdf
PSF-95168-500-Lot024893.pdf
PSF-95166-500-Lot024085.pdf
PSF-95168-100-Lot023967.pdf
PSF-95168-500-Lot023968.pdf
PSF-95166-500-Lot021890.pdf
PSF-95166-500-Lot021891.pdf
PSF-95166-500-Lot022969.pdf
PSF-95167-100-Lot022422.pdf
PSF-95167-500-Lot022423.pdf
PSF-95166-01K-Lot66177249.pdf
PSF-95166-100-Lot66177251.pdf
PSF-95166-500-Lot66177250.pdf
PSF-95166-Lot66177071.pdf
PSF-95166-Lot66177247.pdf
PSF-95166-Lot66177248.pdf
PSF-95167-100-Lot66177066.pdf
PSF-95168-01K-Lot2366176891.pdf
PSF-95168-100-Lot2366176892.pdf
PSF-95167-100-Lot66176047.pdf
PSF-95168-100-Lot66176892.pdf
PSF-95166-01K-Lot66175075.pdf
PSF-95166-100-Lot66175073.pdf
PSF-95166-500-Lot66175074.pdf
PSF-95166-500-Lot66175258.pdf
PSF-95166-Lot66175077.pdf
PSF-95166-Lot66175146.pdf
PSF-95168-100-Lot66174436.pdf
PSF-95168-500-Lot66174440.pdf
PSF-95168-Lot66174764.pdf
PSF-CMK0014-Lot2366174167.pdf
PSF-95166-01K-Lot66173394.pdf
PSF-95166-Lot66171988.pdf
PSF-95166-Lot66171990.pdf
PSF-95168-500-Lot66171763.pdf
PSF-95168-Lot66172088.pdf
PSF-95166-01K-Lot2366171685.pdf
PSF-95166-500-Lot2366171686.pdf
PSF-95166-Lot2366171988.pdf
PSF-95166-Lot2366171990.pdf
PSF-95168-500-Lot2366171763.pdf
PSF-95168-Lot2366172088.pdf
PSF-95166-01K-Lot66158192.pdf
PSF-95166-01K-Lot2366165319.pdf
PSF-95166-01K-Lot2366170167.pdf
PSF-95166-01K-Lot66171685.pdf
PSF-95166-500-Lot66171686.pdf
PSF-95168-Lot66169587.pdf
PSF-95168-Lot66170250.pdf
PSF-95168-Lot66171760.pdf
PSF-95168-Lot66171761.pdf
PSF-95166-01K-Lot66168578.pdf
PSF-95166-01K-Lot2366170167.pdf
PSF-95166-100-Lot2366158195.pdf
PSF-95166-100-Lot2366161624.pdf
PSF-95166-100-Lot66164067.pdf
PSF-95166-100-Lot66168582.pdf
PSF-95166-100-Lot2366168670.pdf
PSF-95166-500-Lot66158194.pdf
PSF-95166-500-Lot66163437.pdf
PSF-95166-500-Lot66168579.pdf
PSF-95166-500-Lot2366168674.pdf
PSF-95166-500-Lot66170168.pdf
PSF-95166-Lot2366158389.pdf
PSF-95166-Lot66160396.pdf
PSF-95166-Lot66170170.pdf
PSF-95167-100-Lot66158250.pdf
PSF-95167-100-Lot2366161697.pdf
PSF-95167-100-Lot66165382.pdf
PSF-95168-100-Lot66160010.pdf
PSF-95168-500-Lot2366168576.pdf
PSF-95168-500-Lot66169586.pdf
PSF-95168-Lot66158255.pdf
PSF-95168-Lot2366159684.pdf
PSF-95168-Lot66168785.pdf
PSF-95166-01K-Lot2366162244.pdf
PSF-95166-01K-Lot66163389.pdf
PSF-95166-01K-Lot66163980.pdf
PSF-95166-01K-Lot66165048.pdf
PSF-95166-01K-Lot2366165319.pdf
PSF-95166-01K-Lot66165565.pdf
PSF-95166-01K-Lot66167425.pdf
PSF-95166-01K-Lot66168091.pdf
PSF-95166-01K-LOT66170167.pdf
PSF-95168-LOT66159684.pdf
PSF-95167-100-LOT66161697.pdf
PSF-95166-01K-LOT66162244.pdf
PSF-95166-100-LOT66161624.pdf
Click here to see all CofA (PSFs)

SDSs
SDS 95166-01K UltraPlex 1-Step ToughMix (4X) 1000R
SDS 95166-100 UltraPlex 1-Step ToughMix (4X), 100R
SDS 95166-10K UltraPlex 1-Step ToughMix (4x) 10000R
SDS 95166-500 UltraPlex 1-Step ToughMix (4X), 500R
SDS 95167-100 UltraPlex 1-Step ToughMix ROX (4X),100R
SDS 95168-01K ULTRAPLEX 1-STEP TM LOW ROX (4X)
SDS 95168-100 UltraPlex 1-Step ToughMix, Low ROX (4X), 100R
SDS 95168-10K UltraPlex 1-Step ToughMix low ROX (4x) 10000R
SDS 95168-500 UltraPlex 1-Step ToughMix, Low ROX (4X), 500R

Publications

Customer Testimonials

UltraPlex 1-Step ToughMix

"In side-by-side comparison, QuantaBio UltraPlex 1-Step ToughMix out performed ABI TaqPath, ABI TaqMan FastVirus, and ABI AgPath-ID One-Step RT-PCR kits."

Sladana B. | Plant Disease Diagnostician
UltraPlex 1-Step ToughMix

"Compared to other 1-step qRT-PCR mixes we have used, UltraPlex 1-Step ToughMix (4X) Low-ROX, 500R performs with the same sensitivity when testing using SARS-CoV-2-spiked saliva samples, but is easier to use and at a significantly lower price per reaction. The mix requires little to no time to thaw, decreasing the time required when preparing samples. The mix is also conveniently aliquoted into multiple tubes, reducing the chance for contamination as opposed to a single, high volume aliquot. Overall, these factors make this a better overall product as compared to competing companies (e.g., Thermo Fisher Scientific or Zymo) and we will continue to use this moving forward."

Rhoel R. D., PhD, MPH Professor of Infectious Diseases UF Eminent Scholar Director | CDC Southeastern Regional Center of Excellence for Vector Borne Diseases

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  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™
  • Agilent AriaMx
  • Douglas Scientific IntelliQube®
  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™
  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
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  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5
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