Extracta Plus DNA
- High yield – Obtain large quantities of high quality DNA, free from PCR inhibitors
- Versatility in sample typesl – Rapid purification of fresh or frozen tissue, cells, blood or bacteria
- Lab friendly – Silica based column eliminates toxic organic chemicals such as phenol/chloroform
- Downstream compatibility – Ideal for sensitive applications such as endpoint PCR, qPCR and next generation sequencing
Extracta Plus DNA Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
Description
Details
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Volume |
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Component Description |
10 Reactions |
50 Reactions |
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Proteinase K (1.25 ml/2) |
1 x 1.25 ml |
1 x 1.25 ml |
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Buffer EPDTL |
1 x 1.8 ml |
1 x 14 ml |
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Buffer EPDL |
1 x 2 ml |
1 x 12 ml |
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Buffer EPDW1 |
1 x 5 ml |
1 x 19 ml |
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Buffer EPDW2 |
1 x 5 ml |
1 x 13 ml |
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Buffer EPDE |
1 x 2 m |
1 x 15 ml |
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Extracta Plus Spin Column |
10 piece |
50 piece |
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Collection Tubes 2 ml |
20 piece |
100 piece |
Performance Data
Extracta Plus DNA complete workflow
This procedure can be completed in just 15 mins, following sample pre-treatment, resulting in ready-to-use DNA for sensitive downstream applications such as endpoint PCR, qPCR and NGS.
Extracted gDNA suitable for qPCR
gDNA was extracted from HEK293 cells using the Extracta Plus DNA Kit (blue) and compared to commercially available high quality gDNA (orange). Serial dilutions from 30 ng – 30 pg were prepared and analyzed by qPCR using PerfeCTa SYBR Green FastMix, Low ROX. Linear amplification of diluted samples demonstrates high quality DNA input. The lack of shift in Cq values indicates the absence in interfering inhibitors in the extracted samples.
Extracted gDNA suitable for qPCR
gDNA was extracted from mouse whole blood (100 μl) and HEK293 cells (1 x 106 cells) using the Extracta Plus DNA Kit. Illumina NGS libraries were prepared from 100 ng of extracted DNA and an equivalent quantity of commercially available high-quality DNA using the sparQ DNA Frag & Library Prep Kit. TapeStation electrophoresis analysis demonstrates a similar fragmentation pattern and yield for all samples, indicating suitability for NGS library preparation.
Extracted gDNA of high molecular weight
The Extracta Plus DNA Kit was used to extract DNA from a variety of tissues: rat (brain and lung), pig (mammary gland), cow (kidney), mouse (liver, testis and tail snip) and guinea pig (stomach and ovary). Samples were digested with EcoRI and visualized by agarose gel electrophoresis. M: Marker, HindIII digest of lambda DNA.
High molecular weight gDNA supports long range PCR.
gDNA was extracted from rat kidney or liver samples and 6, 8, 12 or 20 kb targets were amplified using repliQa HiFi ToughMix. Analysis of PCR products by agarose gel electrophoresis demonstrated clean product for each target, comparable to that of commercially available high quality rat gDNA. The yields are similar, indicating high molecular weight and high quality, free of damage, nicks, etc.
High yielding gDNA extraction
High yielding extraction of total DNA (e.g. genomic, mitochondrial and pathogen) from various tissues as well as cultured cells and blood samples.
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