Whip your experiments into shape with the toughest mix around

Experience the Amplification Difference. ToughMix^®.

Quantabio ToughMix chemistry is the trade secret difference to our mastermix formulations. It has helped scientists for more than 20 years to amplify and analyze challenging samples by overcoming common PCR inhibitors. Widely used across a multitude of molecular biology applications, ToughMix formulations can be used directly from crude lysates as well as following an extraction protocol.

What can ToughMix do for you

Work directly with crude lysates
Avoid expensive and time-consuming purification steps
Compatible with a wide range of probe designs and detection chemistries
High quality Taq polymerase – free of residual host DNA
Neutralize problem causing inhibitors present in crude samples

Overcome many common PCR inhibitors

Inhibitors	Common Source	Reagent performance
		Competitor	ToughMix
Polyphenols	Plant extracts	–
Humic acid	Soil Plant tissues	–
Hematin	Dried bloods Blood spots	–
Hemoglobin	Blood
Polysaccharides	Feces Plant tissues	–
Melanin	Hair Skin	–

Engineered Application Specific Enzymes

Optimized Tough Reagent

Superior Product Performance

ToughMix technology is available for different applications:

Conventional PCR

	repliQa HiFi ToughMix	AccuStart II PCR ToughMix
Features
Concentration	2x	2x
Kit Format	1 tube	1 tube mix + 1 tube loading dye
Amplicon Length	Up to 24kb	Up to 4kb
Applications
	High fidelity PCR, Crude PCR, Fast PCR, Long PCR, High Yield, NGS	Routine PCR, Crude PCR
Available Formats
Master Mix
Packaging (Rxns/Units)	25 Rxns 100 Rxns 500 Rxns	100 Rxns 800 Rxns 4000 Rxns
	Place holder sample request	Place holder sample request

RT-PCR SYBR-based qPCR Probe-based qPCR RT-qPCR

ToughMix technology is available for different applications:

	Conventional PCR		RT-PCR	SYBR-based qPCR	Probe-based qPCR		RT-qPCR
	repliQa HiFi ToughMix	AccuStart II PCR ToughMix	qScript XLT 1-Step RT-PCR Kit	AccuStart Genotyping ToughMix	PerfeCTa qPCR ToughMix	PerfeCTa Muliplex qPCR ToughMix	qScript XLT 1-Step RT-qPCR ToughMix	UltraPlex 1-Step ToughMix	qScript 1-Step Virus ToughMix
Features
Concentration	2x	2x	2x	2x	2x	5x	2x	4x	2x
Kit Format	1 tube	1 tube mix + 1 tube loading dye	2 tubes	1 tube	1 tube	1 tube	1 tube	1 tube	1 tube
Multiplex Compatibility				Up to 2 targets	Up to 2 targets	Up to 5 targets	Up to 4 targets	Up to 5 targets	Up to 4 targets
Amplicon Length	Up to 24kb	Up to 4kb	Exceeding 4kb RNA				70 – 300 bp	70 – 300 bp	70 – 300 bp
Applications
	High fidelity PCR, Crude PCR, Fast PCR, Long PCR, High Yield, NGS	Routine PCR, Crude PCR	High fidelity PCR, Crude PCR	SNP genotyping, high yield	Gene expression, inhibitor tolerant, high yield	Gene expression, inhibitor tolerant, high yield	Inhibitor tolerant, high yield	Inhibitor tolerant, high yield	Inhibitor tolerant, high yield
Available Formats
Master Mix	•	•		•	•	•	•	•	•
Packaging (Rxns/Units)	25 Rxns 100 Rxns 500 Rxns	100 Rxns 800 Rxns 4000 Rxns	20 Rxns 200 Rxns	1250 Rxns 5000 Rxns	250 Rxns 1250 Rxns 5000 Rxns	250 Rxns 1000 Rxns 5000 Rxns	100 Rxns 500 Rxns 2000 Rxns 5000 Rxns	100 Rxns 500 Rxns 1000 Rxns 10000 Rxns	100 Rxns 500 Rxns 2000 Rxns
Place holder sample request	Place holder sample request	Place holder sample request	Place holder sample request	Place holder sample request	Place holder sample request	Place holder sample request	Place holder sample request	Place holder sample request	Place holder sample request

Whip your experiments into shape with the toughest mix around

Experience the Amplification Difference. ToughMix®.

What can ToughMix do for you

Overcome many common PCR inhibitors

ToughMix technology is available for different applications:

Product Finder

Select Your Assay

Starting Template

Assay Format

Detection Chemistry

Multiplexing (more than 3 targets)

Is gene-specific priming (GSP) required?

What current Reverse Transcriptase or cDNA kit are you using?

Select the group which contains your real-time PCR cycler

Choose your application from the categories below

Products

Experience the Amplification Difference. ToughMix^®.