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AccuStart II PCR Genotyping Kit

Completely reagent-based system enables reliable PCR genotyping with minimal pipetting skill
Features & Benefits
  • Simplified, completely reagent-based system requires minimal pipetting skill
  • Premixed electrophretic mobility loading dye reduces chances for post-PCR cross contamination
  • Stabilized 2X PCR SuperMix enables convenient room-temperature setup and is unaffected by repetitive freeze-thaw (>20X)
  • High-yielding, ultrapure modified Taq DNA polymerase delivers robust, reliable duplex assay performance
  • Stringent, ultrapure antibody hotstart ensures sensitive and specific target amplification

 

AccuStart II PCR Genotyping Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Product
Kit Size
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Product
Kit Size
Order Info
Price
Quantity

AccuStart II PCR Genotyping Kit

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Kit Size:
Part Number:
Price:
Quantity:
Kit Size:  100 x 25 μL rxns (6)
Part Number:  95135-100
120.00
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Kit Size:  500 x 25 μL rxns
Part Number:  95135-500
496.00
Add to Cart
To place your order through VWR, click here

Description

The AccuStart II Genotyping Kit is a complete reagent kit designed to support conventional, end-point PCR-based screening of transgenic animal models commonly used in life science research and is validated for use with mouse, fish, or insect tissue specimens. It combines a rapid, 2-component DNA extraction reagent with a user-friendly 2X concentrated PCR SuperMix with loading dye for seamless gel electrophoresis analysis. qPCR-grade genomic DNA template is obtained with minimal extraction volumes (? 100uL) and can be carried out in ? 30-minutes on a standard PCR thermal cycler.
What Customers Say

We have tested several different PCR Mastermixes for genotyping in the past four months. Accustart II was the most convincing one concerning quality and price.”

Medical University of Vienna
Research Scientist
Details

Details

Contents
  • Extracta® DNA Prep for PCR (95091-02)
    • Extraction Reagent
    • Stabilization Buffer
  • AccuStart II GelTrack PCR SuperMix (95136-500)
    • 2X concentrated SuperMix containing optimized concentrations of molecular-grade MgCl2, dNTP blend, AccuStart II Taq DNA Polymerase, reaction buffer, stabilizers and electrophoretic mobility dyes (4kb & 50bp).
Performance Data

Performance Data

AccuStart II Results more specific
Resource Panel
Customer Testimonials

Customer Testimonials

AccuStart II Genotyping Kit

"The quality of this product is amazing, especially for the enzyme mixture. It can successfully amplify most of the genotyping targets in a very short time frame."

Yinghua L. |
AccuStart II Genotyping

"With AccuStart II kit we were able to decrease costs, increase yield of our PCR sensitivity while decreasing and optimizing time. It made genotyping much easier, faster, and cheaper for our multiple transgenic colonies."

Laboratory Manager | H. Lee Moffitt Cancer Center

Details

Contents
  • Extracta® DNA Prep for PCR (95091-02)
    • Extraction Reagent
    • Stabilization Buffer
  • AccuStart II GelTrack PCR SuperMix (95136-500)
    • 2X concentrated SuperMix containing optimized concentrations of molecular-grade MgCl2, dNTP blend, AccuStart II Taq DNA Polymerase, reaction buffer, stabilizers and electrophoretic mobility dyes (4kb & 50bp).

Performance Data

AccuStart II Results more specific

Resources

Product Manuals
Manual AccuStart II Mouse Genotyping

CofA (PSFs)
PSF-95135-100-LOT66161701.pdf
PSF-95135-100-LOT66151673.pdf
PSF-95135-100-LOT66140245.pdf
PSF-95135-100-Lot029828.pdf
PSF-95135-100-Lot027937.pdf
PSf-95135-100-Lot027762.pdf
PSF-95135-100-Lot026681.pdf
PSF-95135-100-Lot025324.pdf
PSF-95135-100-Lot2366161701.pdf
Click here to see all CofA (PSFs)

SDSs
SDS 95135-100 AccuStart II Mouse Genotyping Kit, 100R
SDS 95135-500 AccuStart II Mouse Genotyping Kit, 500R

Publications

Customer Testimonials

AccuStart II Genotyping Kit

"The quality of this product is amazing, especially for the enzyme mixture. It can successfully amplify most of the genotyping targets in a very short time frame."

Yinghua L. |
AccuStart II Genotyping

"With AccuStart II kit we were able to decrease costs, increase yield of our PCR sensitivity while decreasing and optimizing time. It made genotyping much easier, faster, and cheaper for our multiple transgenic colonies."

Laboratory Manager | H. Lee Moffitt Cancer Center

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