AccuStart II PCR SuperMix
| AccuStart II GelTrack PCR SuperMix | sparQ HiFi PCR Master Mix | AccuStart II PCR ToughMix | repliQa HiFi ToughMix | AccuStart Long Range SuperMix | |
---|---|---|---|---|---|---|
Features | ||||||
Concentration | 2x | 2x | 2x | 2x | 2x | 4x |
Amplicon Size | Up to 4 kb | Up to 4 kb | Up to 4 kb | Up to 4 kb | Up to 24 kb | Up to 24 kb |
Extension Time* | 60 sec/kb | 60 sec/kb | 30 sec/kb | 60 sec/kb | 1 - 10 sec/kb | 30 - 60 sec/kb |
Multiplex PCR | Up to 6 Targets | |||||
Fidelity vs. Taq | 1x | 1x | 80x | 1x | 90x Taq | 10 - 12x Taq |
dU Tolerant | No | No | No | No | Yes | Yes |
5’ → 3’ exo | x | x | N/A | x | N/A | N/A |
3’ → 5’ exo | N/A | N/A | x | N/A | x | x |
Resulting Ends | 3’ dA overhang | 3’ dA overhang | Blunt | 3’ dA overhang | Blunt | Blunt/T overhangs |
Applications | ||||||
Routine PCR | x | x | x | x | x | |
High Fidelity | x | ** | x | |||
Crude PCR | x | ** | ||||
Fast PCR | ** | |||||
Long PCR | x | ** | ||||
High Yield | ** | x | ||||
NGS Library Amplification | ** | x | ||||
Available Formats | ||||||
Hot Start | x | x | x | x | x | x |
Master Mix | x | x | x | x | x | x |
Loading Dye Available | x | x | ||||
Packaging (Rxns/Units) | 100 Rxns 500 Rxns 4000 Rxns | 100 Rxns 500 Rxns 4000 Rxns | 50 Rxns 250 Rxns | 100 Rxns 800 Rxns 4000 Rxns | 25 Rxns 100 Rxns 500 Rxns | 25 Rxns 100 Rxns |
PCR Products
Showing 6–8 of 8 results
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qScript XLT 1-Step RT-PCR Kit
The qScript XLT One-Step RT-PCR Kit is a convenient and highly sensitive 2-reagent system for amplification of complex RNA templates exceeding 4 kb in length. Both enzyme incubation sequences are carried out in the same reaction mixture without opening between… -
repliQa HiFi ToughMix
The repliQa HiFi ToughMix is a 2x, ready-to-use master mix that contains all the components for high fidelity PCR amplification, including a genetically modified DNA polymerase coupled with hot start antibodies. This unique, next generation master mix provides 90x higher…